-A +A

imprimer la page

Solution structure of the Nereis diversicolor Sarcoplasmic Calcium-binding Protein

Solution structure of the <i>Nereis diversicolor</i> Sarcoplasmic Calcium-binding Protein

Solution structure, obtained by NMR spectroscopy, of the Nereis diversicolor Sarcoplasmic Calcium-binding Protein in Ca2+-bound form (Craescu C. T., unpublished results). In absence of metal ion (Ca2+ or Mg2+) the protein is a molten globule and binding of the first ion induces formation of the bound structure [1]. Structural stability is strongly dependent on the presence and type of bound metal ion [2].

[1] Prêcheur, B., et al., Nereis sarcoplasmic Ca2+-binding protein has a highly unstructured apo state which is switched to the native state upon binding of the first Ca2+ ion. FEBS Letters (1996), 395, 89-94.

[2] Christova, P., J. Cox, A., and C. Craescu, T., Ion-induced conformational and stability changes in Nereis sarcoplasmic calcium binding protein: evidence that the apo state is a molten globule. Proteins:Structure, Function, and Genetics (2000), 40(2), 177-184.